Post-Doc_positions_Nantes

1-RNA-Therapeutic in vivo Efficiency (18-months, start date: 1st June)

KEY RESPONSABILITIES:

• Design and conduct in vivo experiments in multiple species via collaborative partnerships to evaluate novel mRNA designs and supramolecular formulations
• Select and design mRNA-encoded antibodies to support high in vivo therapeutic antibody secretion
• Lead multistep experimental designs including planning and execution to assess safety and mRNA expression after in vivo injection
• Perform cell based neutralizing antibody assay, innate/adaptive immune profiling and pharmacodynamics assays using technologies such as ELISA, MSD
• Investigate structural and biochemical modifications that enhance mRNA expression while reducing immune processing of mRNA.
• Prepare figures and authors manuscripts for submission to high-impact journals

 

BASIC QUALIFICATIONS

• Ph.D in Cell Biology, Immunology, nucleic acids-based vaccines or therapy or related field with 2-4 years of experience, demonstrated by a strong track record of peer-reviewed publications and project achievements
• Certification in animal experimentation-Project designer
• Previous experience with small animal models relevant to RNA delivery
• Experience with single and multiplex assays including MSD platform

 

2-RNA-Synthetic delivery systems (18-months, start date: 1st June)

KEY RESPONSABILITIES:

• Lead medicinal chemistry efforts aimed at optimizing a new class of synthetic delivery systems for RNA therapeutics
• Identify and develop suitable analytical methods for characterization and validation
• Oversee chemistry synthesis activities at CRO/CDMO partners, including approval of protocols and reports
• Collaborate with the team to evaluate and optimize the efficiency and safety of our RNA delivery technologies
• Prepare technical updates for reporting to funding agencies
• Prepare figures and authors manuscripts for submission to high-impact journals

 

BASIC QUALIFICATIONS:

• Ph.D in chemistry with 2-4 years of experience, demonstrated by a strong track record of peer-reviewed publications and project achievements
• Experience in polymer chemistry is preferred; experience in lipid and nucleotide is a plusimentation-Project designer
• Strong synthetic chemistry expertise and proficiency in analytical techniquesng track record of peer-reviewed publications and project achievements

 

3-RNA-Directed antibody in vitro design (12-months, start date: 1st June)

KEY RESPONSABILITIES:

• Lead mRNA element engineering efforts to optimize novel mRNA structure designs for RNA therapeutics achieving unprecedented levels of expression and stability
• Design and execute all-based assays in human and rodent cell lines to characterize the efficacy and safety of novel RNA therapeutic leads.
• Develop cell based neutralizing antibody assays against various viral targets
• Design RNA formulation using adjunct regulatory elements to modulate antibody secretion
• Investigate mechanism of action of lead candidates and establish biologically relevant endpoints for mRNA antibody selection and infectious disease applications
• Prepare figures and authors manuscripts for submission to high-impact journals

 

BASIC QUALIFICATIONS:

• Ph.D in Molecular Biology, Biochemistry, Molecular Cell Biology or related field with 2-4 years of experience, demonstrated by a strong track record of peer-reviewed publications and project achievements
• Extensive experience in in vitro cell culture, as well as cellular based assays and analysis techniques (eg flow cytometry, confocal microcopy imaging, PCR, ELISA, western blotting, sequencing…)

 

How to apply?

Candidates should submit a motivation letter, a Curriculum Vitae (including publication list) and contact details of three professional references (including the PhD advisor) to bruno.pitard@univ-nantes.fr