Innovative Nanomolecular Luminescent Probes for Live Detection of Circular RNAs in cellular environment

Grenoble Alpes University – Starting November 2025

Circular RNAs (circRNAs) are emerging biomarkers and therapeutic targets in cancer, yet their detection remains challenging due to their low abundance and unique back-splicing junction. This PhD project aims to develop a biocompatible, near-infrared (NIR) fluorescent nanomolecular system for the selective detection of circRNAs overexpressed in treatment-resistant non-small cell lung cancer (NSCLC). The approach is based on atomically precise gold nanoclusters (AuNCs), which are ultra-small gold nanoparticles (<3 nm), functionalized with antisense oligonucleotides (ASOs) targeting circRNA junctions.

We will engineer and validate AuNC-based genosensors, including beacon-like and multimeric assemblies, to achieve sensitive and specific circRNA detection in live cells by fluorescence microscopy and others optical imaging techniques. The project will also explore on a second stage the real-time imaging in multicellular models. This work will contribute to the development of next-generation tools for precision oncology and liquid biopsy diagnostics.

We invite applications for a fully funded PhD position (3 years) within a highly collaborative and interdisciplinary project jointly developed under PhD program with McMaster University (Canada), in partnership with Prof. N. Hildebrandt, an expert in nanobiophotonics with the possibility of 3-4 months stay in Hildebrandt’s group. The project will be hosted at the Institute for Advanced Biosciences (IAB), Grenoble Alpes University, and will benefit from a strong collaboration with an in-house team specialized in circRNA biology.

The successful candidate will receive training in a wide range of techniques at the interface of nanochemistry, biomolecular interactions, and bioimaging. The project aims to develop innovative biosensing strategies by integrating nanobiophotonic tools with RNA biology, with a particular focus on the detection and functional analysis of circRNAs.

Candidate Profile

We are seeking a highly motivated candidate with a Master’s degree (or equivalent) in nanosciences, biotechnology, biophysics, or a closely related discipline. A strong background in physical chemistry, bioanalytical chemistry, or nanobiochemistry is expected. Prior experience in biosensing, fluorescence techniques, or RNA biology will be considered a plus. The candidate should have a strong interest in interdisciplinary research at the crossroads of nanotechnology, molecular biology, and biosensing.

How to Apply

Please send the following documents in a single PDF file:

A brief cover letter describing your research interests and motivation

Your CV

A copy of your academic transcripts

One to two reference letters or names of referees

Applications and inquiries should be sent to:

Dr. Xavier Le Guével

Email: xavier.le-guevel@univ-grenoble-alpes.fr